Built on BEHAV3D — Nature Biotechnology, 2023

Understand How
Immune Cells Behave

Current assays tell you whether tumor cells die. CellWorkflow tells you why. Track individual T cells through 3D organoid co-cultures, classify their behavioral phenotypes, and quantify killing efficiency — all from your browser.

Organoid Co-Culture Behavioral Analysis Tracking + Classification + Quantification Serial Killer Engager Scanner Inactive Active Behavioral Phenotypes

Immunotherapy Analysis Is Stuck in the Last Decade

Live-cell imaging can reveal how immune cells behave in tumors. But the tools to analyze this data haven't kept pace.

90%
Fail Rate
$500M+
Per Therapy

Binary Assays Miss What Matters

Current immunotherapy assays measure a single outcome: did the tumor shrink? They tell you nothing about why a therapy works in one patient and fails in another. The functional behaviors that drive response or resistance remain invisible.

5D
Datasets
TB-scale
Per Study

Data Exists, but Analysis Doesn't Scale

Organoid co-cultures and live-cell imaging can capture immune-tumor interactions in 3D over time. But analyzing terabyte-scale 5D datasets requires computational expertise that most immunotherapy labs and pharma teams lack.

Weeks
Per Dataset
3+
Tools Required

Fragmented Tools, Non-Reproducible Results

Researchers cobble together ImageJ, Python scripts, and manual annotation. Each lab develops its own ad-hoc pipeline. Results vary between analysts, experiments are hard to reproduce, and scaling to screening is impossible.

From Imaging to Behavioral Insight in 3 Steps

Upload your co-culture imaging data. CellWorkflow handles the rest.

1

Upload Co-Culture Data

Drag and drop your organoid co-culture imaging files directly into the browser. TIFF, ND2, CZI, and more are automatically converted for efficient cloud streaming. Terabyte-scale 5D datasets ready in minutes.

2

Build Your Analysis Pipeline

Connect visual nodes for T-cell segmentation, tracking, and behavioral classification. Adapt AI models to your imaging setup without writing code. Every step runs on cloud GPUs with instant previews.

3

Extract Behavioral Phenotypes

CellWorkflow classifies each tracked cell by behavior: serial killers, engagers, scanners, and more. Export motility metrics, killing efficiency, and engagement statistics as publication-ready figures or CSV data.

Built for Immunotherapy Research

3D tracking, behavioral classification, and customizable AI models in a single cloud-native workflow.

3D Cell Tracking ID: 12 ID: 47 ID: 83 ID: 156 ID: 7 ID: 201 Tracked Cells 247 cells tracked 189 active tracks Tracking Metrics 45 Z-Slices 120 Timepoints Time Range 0h — 72h 15 min intervals Timeline Frame 60 / 120 — 36h elapsed Play Export
Behavioral Classification — UMAP 247 cells classified UMAP 1 UMAP 2 Serial Killers Engagers Scanners Inactive Behavioral Distribution Serial Killers 15% Engagers 25% Scanners 30% Inactive 20% Unclassified 10% Key Metrics 3.2 targets/cell (serial killers) 42 min mean contact (engagers) Export Report
Fine-tune Model No Code Required BASE MODEL Cellpose - cyto2 TRAINING DATA Drop images here or browse 12 images uploaded ANNOTATIONS 247 labeled cells EPOCHS 50 ~15 min Training Progress 60% Training... VALIDATION METRICS 0.94 IoU Score 0.91 F1 Score 98.2% Cell Detection Start Fine-tuning Available Models C Cellpose Cell & nuclei segmentation S StarDist Star-convex object detection S SAM Segment Anything Model U Ultrack Multi-hypothesis tracking N N2V Denoising Self-supervised denoising + More models coming soon

3D Organoid Visualization

Render immune-tumor co-cultures in full 3D directly in your browser. Navigate Z-stacks, time-lapses, and multiple channels at terabyte scale.

Automated Cell Tracking

Track hundreds of individual immune cells across time-lapse experiments. Handles cell divisions, contacts, and complex 3D trajectories.

Behavioral Phenotyping

Classify tracked cells into functional states: serial killers, engagers, scanners, and more. Quantify killing efficiency and contact dynamics.

Cloud GPU Processing

Process 5D datasets on scalable cloud GPUs. No workstation upgrades needed. Results in hours, not weeks.

Customizable AI Models

Adapt pre-trained segmentation models to your specific organoid setup, imaging modality, and cell types. No coding required.

Reproducible Pipelines

Define standardized analysis workflows that every lab member follows. Version-controlled results for multi-site studies and regulatory submissions.

Validated Science. Published Methods.

CellWorkflow is built on BEHAV3D, a methodology validated in leading journals and adopted by major pharmaceutical companies.

Nature Biotechnology
BEHAV3D: T cell behavior in patient cancer organoids
2023 · 127 citations
Nature Protocols
3D live imaging analysis platform
2024
Nature Protocols
Behavior-guided transcriptomics
2025
Nature Reviews Cancer
Live-cell imaging in cancer treatment
2023 · Invited Review

Why CellWorkflow?

What changes when behavioral analysis becomes accessible.

Beyond binary readouts

See how immune cells actually behave in tumors, not just whether tumor cells die. Identify serial killers, measure engagement dynamics, and quantify killing efficiency per cell.

No computational expertise needed

Drag-and-drop workflows replace Python scripts and ImageJ macros. Any immunotherapy researcher can run BEHAV3D-level analysis.

Screening-compatible scale

Process hundreds of co-culture experiments in parallel on cloud GPUs. Turn behavioral analysis from a boutique capability into a screening tool.

Reproducible across labs

Standardized pipelines ensure consistent results regardless of who runs the analysis. Critical for multi-site studies and regulatory submissions.

Hours instead of weeks

Automated tracking and classification replace manual annotation. What took a computational biologist weeks now takes an afternoon.

Adapts to your setup

Fine-tune AI models on your specific organoid system, imaging modality, and cell types. Works with CAR-T, bispecifics, CAR-NK, and more.

Understand How Your Immunotherapy Works

Move beyond binary kill assays. CellWorkflow reveals the behavioral mechanisms that determine whether immunotherapy succeeds or fails. Request access to start analyzing your co-culture imaging data.